Journal: Immunology

Article Title: Protective B-cell epitopes of Francisella tularensis O -polysaccharide in a mouse model of respiratory tularaemia

doi: 10.1111/j.1365-2567.2012.03589.x

Figure Lengend Snippet: FB11 and Ab52 confer survival to Francisella tularensis LVS-infected BALB/c mice in a dose-dependent manner. Five- to six-week old BALB/cJ female mice (n = 4–6) were inoculated intranasally with 5 × 104 to 7 × 104 colony-forming units (CFU) of LVS and injected intraperitoneally 1 hr later with graded doses of FB11 or Ab52. Survival was monitored for 28 days, and data from three experiments were combined. The log rank test was used to compare monoclonal antibody (mAb) -treated groups with vehicle groups. **P < 0·001; ***P < 0·0001. No difference in survival was observed between the groups treated with the 6 μg mAb dose or vehicle control.

Article Snippet: 39 BALB/c mouse serum and protein A-purified IgG (both sterile, without preservative) were purchased from Innovative research (Novi, MI).

Techniques: Infection, Injection

Journal: Immunology

Article Title: Protective B-cell epitopes of Francisella tularensis O -polysaccharide in a mouse model of respiratory tularaemia

doi: 10.1111/j.1365-2567.2012.03589.x

Figure Lengend Snippet: FB11 and Ab52 reduce bacterial burden in BALB/c mice infected intranasally with Francisella tularensis LVS. Three groups of 19 BALB/cJ female mice, 5–6 weeks of age, were used in this experiment; seven mice were pre-assigned for survival (a) and the remaining mice were pre-assigned for determination of bacterial burden (b). Mice were inoculated intranasally with 7 × 104 colony-forming units (CFU) of LVS and 1 hr later, injected intraperitoneally with 50 μg FB11 or Ab52, or with PBS only. Survival was monitored for 28 days (a). For determination of bacterial burden, mice were euthanised on days 0 (1 hr after bacterial inoculation), 1, 3, 6, 14 and 28. For each time-point in B, three mice per group (except for the Ab52 group on day 14, when n = 2, and the PBS groups on days 14 and 28, when there were no surviving mice) were used for CFU determination, and means and standard deviations were calculated. Student’s t-test statistical analysis was performed and significant differences from the PBS control are indicated for each monoclonal antibody by color code: FB11, black, top; and Ab52, grey, bottom. *P < 0·05; **P < 0·005; ***P < 0·0005.

Article Snippet: 39 BALB/c mouse serum and protein A-purified IgG (both sterile, without preservative) were purchased from Innovative research (Novi, MI).

Techniques: Infection, Injection

Journal: Immunology

Article Title: Protective B-cell epitopes of Francisella tularensis O -polysaccharide in a mouse model of respiratory tularaemia

doi: 10.1111/j.1365-2567.2012.03589.x

Figure Lengend Snippet: FB11 and Ab52, administered 2 hr or 19 hr after intranasal infection of BALB/c mice with Francisella tularensis SchuS4, prolong survival. BALB/cJ female mice, 7–8 weeks of age, were inoculated intranasally with SchuS4 and injected intraperitoneally with the indicated monoclonal antibody (mAb) doses or with Vehicle at the indicated times after infection. The log-rank test was used to compare mAb-treated groups with Vehicle-treated groups. P-values are indicated by colour code: FB11, black; Ab52, grey. (a) Combined results from four experiments for Ab52 [n = 22 (7 + 5+5 + 5)], only the first two of which included FB11 [n = 12 (7 + 5)]; the SchuS4 inocula for the four experiments were 20 colony-forming units (CFU), 25 CFU, 52 CFU and 57 CFU. (b) Ab52 at 30 or 100 μg (n = 5) tested in the same (57 CFU) experiment shown in (a). (c,d) FB11 and Ab52 at 10 μg (n = 5) or 50 μg (n = 5 for FB11, n = 10 for Ab52) tested 19 hr after infection with 35 CFU SchuS4.

Article Snippet: 39 BALB/c mouse serum and protein A-purified IgG (both sterile, without preservative) were purchased from Innovative research (Novi, MI).

Techniques: Infection, Injection

Journal: Immunology

Article Title: Protective B-cell epitopes of Francisella tularensis O -polysaccharide in a mouse model of respiratory tularaemia

doi: 10.1111/j.1365-2567.2012.03589.x

Figure Lengend Snippet: FB11 and Ab52 reduce bacterial burden in BALB/c mice infected intranasally with Francisella tularensis SchuS4. Groups of BALB/c mice (n = 2 in a and 5 in b), 7–8 weeks of age, were infected intranasally with 116 colony-forming units (CFU) (a) or 164 CFU (b) of SchuS4 and injected intraperitoneally with 50 μg Ab52, FB11 or CO17-1A (IgG2a isotype control), 2 hr later. Mice were euthanised on day 3 after bacterial infection. Bacterial burdens in spleen and blood were determined by plating teased spleen suspension or heparinized blood on chocolate agar plates for bacterial enumeration (CFU per spleen or per ml blood). The difference between the Ab52 or FB11 and control (CO17-1A) groups was analysed by Student’s t-test. P < 0·05 is indicated by asterisk. Bars denote standard deviation from the means.

Article Snippet: 39 BALB/c mouse serum and protein A-purified IgG (both sterile, without preservative) were purchased from Innovative research (Novi, MI).

Techniques: Infection, Injection, Standard Deviation

Journal: Cell reports

Article Title: Staphylococcus aureus exploits lipoic acid salvage to combat host oxidative stress

doi: 10.1016/j.celrep.2025.116095

Figure Lengend Snippet: (A) Bacterial recovery of the indicated strains from the kidneys and peritoneal cavity of Swiss Webster mice 24 h after intraperitoneal injection ( n = 24). * p < 0.05 and ***, p < 0.001, calculated by the Kruskal-Wallis test with Dunn’s multiple comparisons test. (B) Bacterial recovery of the indicated strains from the kidneys of C57BL/6 mice 24 h after bloodstream infection ( n = 16). * p < 0.05 and ** p < 0.01 by Kruskal-Wallis test with Dunn’s multiple comparisons test. (C) Bacterial recovery of the indicated strains from the peritoneal cavity of C57BL/6 and Cybb − mice 24 h after intraperitoneal injection with 1 × 10 8 CFUs ( n = 20) or 1 × 10 7 CFUs ( n = 10). ** p < 0.01, *** p < 0.001 by unpaired t test (1 × 10 8 CFUs) or Mann-Whitney test (1 × 10 7 CFUs). (D) Outgrowth of the indicated strains after infection with isolated macrophages from Swiss Webster mice. (E) Outgrowth of the indicated strains after infection with isolated macrophages from Swiss Webster mice that were treated with vehicle control (water) or the NADPH oxidase inhibitor gp91ds-tat (50 μM). (F) Outgrowth of the indicated strains after infection with isolated macrophages from C57BL/6 and Cybb − mice. ** p < 0.01, *** p < 0.001, **** p < 0.0001 by two-way ANOVA with Tukey’s post hoc test (D–F). Data are represented as individual values with the median indicated as a solid line.

Article Snippet: Mouse Swiss Webster serum , Innovative Research Inc , Cat # 50-203-5738.

Techniques: Injection, Infection, MANN-WHITNEY, Isolation, Control

Journal: Journal of Immunotoxicology

Article Title: An ELISA for detection of complement-bound circulating immune complexes in mice

doi: 10.1080/1547691x.2019.1599471

Figure Lengend Snippet: Figure 1. Preparation of the positive control. The positive control was used as quality control in the enzyme-linked immunosorbent assay (ELISA). Mouse serum con- taining mouse immunoglobulin (Ig) and inactivated complement was incubated with heat-aggregated human IgG (“Complement Activator”) to stimulate complement activation by the classical complement pathway. Monomeric mouse IgG was able to bind to aggregated human IgG, leading to formation of complement bound hIgG-mIgG complexes. HRP (horse radish peroxidase) signal.

Article Snippet: In brief, mouse serum complement (Innovative Research, Novi, MI, cat. no IMS-C57BL6-COMPL) was incubated at 37 C for 90min with 1:100 HAGG (Complement Activator, Quidel, San Diego, CA, cat. #A114), followed by inactivation by addition of ice-cold 500mM ethylenediamine-tetraacetic acid (EDTA; to final concentration of 20mM) and storage (undiluted) at 80 C until analysis.

Techniques: Positive Control, Control, Enzyme-linked Immunosorbent Assay, Incubation, Activation Assay